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 Re: إختراق علمي سوداني ألماني في علاج حالا� (Re: Hamad Hamad)
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J Ethnopharmacol. 2015 Jul 10. pii: S0378-8741(15)30022-2. doi: 10.1016/j.jep.2015.07.005. [Epub ahead of print]
Cytotoxicity of 35 medicinal plants from Sudan towards sensitive and multidrug-resistant cancer cells.
Saeed ME1, Abdelgadir H2, Sugimoto Y3, Khalid HE2, Efferth T4.
Author information
1Department of Pharmaceutical Biology, Institute of Pharmacy and Biochemistry, Johannes Gutenberg University, Mainz, Germany.
2Department of Pharmacognosy, University of Khartoum, Khartoum, Sudan.
3Division of Chemotherapy, Faculty of Pharmacy, Keio University, Tokyo, Japan.
4Department of Pharmaceutical Biology, Institute of Pharmacy and Biochemistry, Johannes Gutenberg University, Mainz, Germany. Electronic address: mailto:mailto:mailto:mailto:mailto:[email protected]@uni-mainz.demailto:[email protected]@uni-mainz.demailto:mailto:[email protected]@uni-mainz.demailto:[email protected]@uni-mainz.demailto:mailto:mailto:[email protected]@uni-mainz.demailto:[email protected]@uni-mainz.demailto:mailto:[email protected]@uni-mainz.demailto:[email protected]@uni-mainz.demailto:mailto:mailto:mailto:[email protected]@uni-mainz.demailto:[email protected]@uni-mainz.demailto:mailto:[email protected]@uni-mainz.demailto:[email protected]@uni-mainz.demailto:mailto:mailto:[email protected]@uni-mainz.demailto:[email protected]@uni-mainz.demailto:mailto:[email protected]@uni-mainz.demailto:[email protected]@uni-mainz.de.
Abstract
BACKGROUND:
Cancer is a complex disease with multiple genetic and epigenetic alterations. Since decades, the hallmark of cancer therapy is chemotherapy. Cytotoxic drugs erase rapidly dividing cells without sufficient differentiation between normal and cancerous cells resulting in severe side effects in normal tissues. Recently, strategies for cancer treatment focused on targeting specific proteins involved in tumor growth and progression. The present study was designed to investigate the cytotoxicity of 65 crude extracts from 35 Sudanese medicinal plants towards various cancer cell lines expressing molecular mechanisms of resistance towards classical chemotherapeutics (two ATP-binding cassette transporters, ABCB1 (P-glycoprotein) and ABCB5, tumor suppressor p53, epidermal growth factors receptor (EGFR). And the aim was to identify plant extracts and isolated compounds thereof with activity towards otherwise drug-resistant tumor cells.
METHODS:
Cold maceration was performed to obtain crude extracts from the plants. The resazurin assay was used to determine cytotoxicity of the plant extracts. Microarray-based mRNA expression profiling, COMPARE, and hierarchical cluster analyses were applied to identify, which genes correlate with sensitivity or resistance to ambrosin, the main constituent of the most active extract Ambrosia maritima.
RESULTS:
The results of the resazurin assay on different tumors showed that Lawsonia inermis, Trigonella foenum-graecum and Ambrosia maritma were the most active crude extracts. Ambrosin was selected as one active principle of A. maritima for microarray-based expression profiling. Genes from various functional groups (transcriptional regulators, signal transduction, membrane transporters, cytoskeleton organization, chaperones, immune system development and DNA repair) were significantly correlated with response of tumor cell lines to ambrosin.
CONCLUSION:
The results revealed cytotoxicity and pharmacogenomics studies of Sudanese medicinal plants provide an attractive strategy for the development of novel cancer therapeutics with activity towards cell lines that resistance to established anticancer agents.
Copyright © 2015. Published by Elsevier Ireland Ltd.
KEYWORDS:
Chemotherapy; Cytotoxicity; Microarray analysis; Multidrug resistance; Phytotherapy; Sudanese medicinal plants
PMID: 26165828 [PubMed - as supplied by publisher]
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http://www.researchgate.net/profile/Mohamed_Saeed7http://www.researchgate.net/profile/Mohamed_Saeed7
الاوراق العلمية التي تم نشرها في هذا الخصوص
1- Cytotoxicity of 35 medicinal plants from Sudan towards sensitive and multidrug-resistant Cancer cellsf
2- Activity of the dietary flavonoid, apigenin, against multidrug-resistant tumor cells as determined by pharmacogenomics and molecular docking
3- the lignan sesamin reveals cytotoxicity toward cancer cells: pharmacogenomics determination of genes associated with sensitivity or
(عدل بواسطة Hamad Hamad on 09-01-2015, 04:58 AM)
(عدل بواسطة Hamad Hamad on 09-01-2015, 04:59 AM)
(عدل بواسطة Hamad Hamad on 09-01-2015, 05:06 AM)
(عدل بواسطة Hamad Hamad on 09-01-2015, 05:06 AM)
(عدل بواسطة Hamad Hamad on 09-01-2015, 05:07 AM)
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الكاتب |
Date |
 إختراق علمي سوداني ألماني في علاج حالات سرطانية | نعمات عماد | 08-30-15, 08:59 AM |
| Re: إختراق علمي سوداني ألماني في علاج حالا� | Abureesh | 08-30-15, 09:41 AM |
| Re: إختراق علمي سوداني ألماني في علاج حالا� | نعمات عماد | 08-30-15, 10:06 AM |
| Re: إختراق علمي سوداني ألماني في علاج حالا� | نصار | 08-30-15, 03:48 PM |
| Re: إختراق علمي سوداني ألماني في علاج حالا� | Abureesh | 08-31-15, 05:09 AM |
 Re: إختراق علمي سوداني ألماني في علاج حالا� | مرتضى احمد عبد القادر | 08-31-15, 05:40 AM |
| Re: إختراق علمي سوداني ألماني في علاج حالا� | نصار | 09-01-15, 00:08 AM |
| Re: إختراق علمي سوداني ألماني في علاج حالا� | Abureesh | 09-01-15, 01:14 AM |
| Re: إختراق علمي سوداني ألماني في علاج حالا� | Hamad Hamad | 09-01-15, 04:49 AM |
| Re: إختراق علمي سوداني ألماني في علاج حالا� | Abureesh | 09-01-15, 04:57 AM |
| Re: إختراق علمي سوداني ألماني في علاج حالا� | Hamad Hamad | 09-01-15, 04:57 AM |
| Re: إختراق علمي سوداني ألماني في علاج حالا� | Abureesh | 09-01-15, 05:55 AM |
| Re: إختراق علمي سوداني ألماني في علاج حالا� | نعمات عماد | 09-01-15, 08:58 AM |
| Re: إختراق علمي سوداني ألماني في علاج حالا� | نعمات عماد | 09-01-15, 09:56 AM |
| Re: إختراق علمي سوداني ألماني في علاج حالا� | سيف النصر محي الدين | 09-01-15, 11:44 AM |
| Re: إختراق علمي سوداني ألماني في علاج حالا� | Abureesh | 09-01-15, 03:45 PM |
| Re: إختراق علمي سوداني ألماني في علاج حالا� | Mohamed Mohamed | 09-01-15, 02:07 PM |
| Re: إختراق علمي سوداني ألماني في علاج حالا� | Hamad Hamad | 09-02-15, 01:44 AM |
| Re: إختراق علمي سوداني ألماني في علاج حالا� | نعمات عماد | 09-02-15, 09:01 AM |
| Re: إختراق علمي سوداني ألماني في علاج حالا� | نعمات عماد | 09-02-15, 10:04 AM |
| Re: إختراق علمي سوداني ألماني في علاج حالا� | نصار | 09-13-15, 03:39 PM |
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Hamad Hamad
فيس بوك
تويتر
انستقرام
يوتيوب
بنتيريست