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 Re: Molecular biology (Re: WAD ELGAALI#4)
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left]From what I understand you are attempting to detect a cluster/relationship in a phylogeny. There are several methods to perform that. The organsim you are attempting to relate is a eukaryote My assumption [is that you will be looking at a conserved region within the species to identify any differences in the conserved regions. There are several models to study these differences. and assess what is known as divergence, however the concept remains the same. Therefore: you need to learn about the conserved areas in your organism e.g. Ribosomal RNA or any other functional genes without which the organism cannot function These usually code vital me-tabolic processes . After that I think your experiment will involve some sort of PCR and sequencing. Following that you will use whichever sequence alignment software your group is using There are several models and I suggest you read the following paper by Lawson and Daniel Falush> Population identification using genetic data.
http://www.ncbi.nlm.nih.gov/pubmed/22703172
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